EquiPure

equipure

Density Gradient product designed to increase the quality and viability of equine sperm

Equine Reproduction

Net contents:
100 mL

This is achieved by separating the sperm by density centrifugation prior to insemination, freezing, IVF or semen sexing. EquiPure not only eliminates a high proportion of the abnormal spermatozoa but also removes the source of reactive oxygen species, most bacteria and many viruses (EAV). This significantly increases the sperm survival and their fertilizing potential.

Features

– Improves fertility rates, especially in sub-fertile stallions

 

– Improves freezeability of equine sperm

 

– Removes bacteria and other unwanted components from raw ejaculates or inseminating doses.

 

-Removes Equine Arteritis Virus (EAV) particles

 

-Studies performed on the hygienic aspects of semen storage for AI demonstrate that the addition of antibiotics to extenders is often not enough to eliminate the detrimental influence of bacteria on sperm motility, viability and fertility. EquiPure centrifugation prior to freezing removes bacteria and many viruses (EAV).

 

-Poor quality frozen semen: If the sperm to be treated are already frozen, thaw at 37ºC the straws, and wait for approximately 10 minutes prior centrifugation. Then, put the same volume of EquiPure (proportion 1:1). Example: In a tube of 15 mL, first add 2 mL of EquiPure (Bottom) 4 straws (2 mL) of semen (TOP) centrifuge 400 xg 20 minutes. Finally, resuspend the pellet with 1 to 2 mL of BotuCRIO.

Available

Net contents 100 mL

Video how to use

Reproduzir vídeo

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

BotuDOG TURBO

BotuDog turbo

Fresh and Cooling Semen

Canine Reproduction

Net contents:
1.6 g

Composition

Casein, sugars, preservatives, pentoxifylline, and excipients.

How to prepare

Add all the content of this packet in 20 mL of sterile water. Shake very well.

Recommendation

For transport of cooled semen, use the 2:1, 3:1 or 4:1 dilution (extender: semen) (v/v),
according to concentration of ejaculate.
For best results in cooled semen transport, use BotuFLEX.

Available

Net contents: 1.6 g

Observation

After mixing, store it at 2-8ºC.

Important

Before mixing, store it below 35ºC.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuDOG

BotuDog

Fresh and Cooling Semen

Canine Reproduction

Net contents:
1.6 g

Composition

Casein, sugars, preservatives, and excipients.

How to prepare

Add all the content of this packet in 20 mL of sterile water. Shake very well.

Recommendation

For transport of cooled semen, use the 2:1, 3:1 or 4:1 dilution (extender: semen) (v/v),
according to concentration of ejaculate.
For best results in cooled semen transport, use BotuFLEX.

Available

Net contents: 1.6 g

Observation

After mixing, store it at 2-8ºC.

Important

Before mixing, store it below 35ºC.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuDOG Freezing

BotuDog Freezing

Freezing of Canine Semen

READY TO USE

Canine Reproduction

Net contents:
8 mL

Composition

Sugars, antioxidants, amino acids, egg yolk, cryoprotectant, and vehicle.

Available

Net contents: 8 mL

Storage and stability

Store it in a freezer at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuTURBO

BotuTurbo

Increase sperm motility extender

Developed to improve vigor of slow moving sperm

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuTURBO is an extender developed to improve vigor of slow moving sperm. It is highly recommended for fresh insemination and semen transport from stallions that need increased movement.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, pentoxifylline, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

For transport of cooled semen dilute to concetration of 30 to 50 million sperm per mL. Use 5ºC for semen transport. Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen. High recommended for reinforcement breeding (Thoroughbred).

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

*Consult the Botupharma APP to do the dilution calculations.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuSEMEN SPECIAL

BotuSemen special

Maximum Protection for Sperm

with cholesterol

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSEMEN SPECIAL an extender especially developed for stallions with semen sensitive to cooling process. Its composition is based on the addition of essential lipids to maintain the plasma membrane stability of the sperm during the cooling process.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, cholesterol and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

It is important to respect the dilutions from the table below. After dilution wait 15 minutes before use. In cases that centrifugation before cooling is necessary, resuspend with at least 100x10E6 sptz/mL.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

For better results in freezing semen, use BotuSemen Gold during centrifugation.

SPERMATIC CONCENTRATION
DILUTION (V:V)
SPERMATIC CONCENTRATION
DILUTION (V:V)
90x10E6/mL
1:1 Centrifuged Resuspended 100x10E6
170 to 210x10E6/mL
4:1
90 to 120x10E6/mL
2
He
Helium
130 to 160x10E6/mL
3
Li
Lithium

*Consult the Botupharma APP to do the dilution calculations.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuSEMEN GOLD

BotuSemen gold

High Fertility Semen Extender

casein + cholesterol

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSEMEN GOLD has been developed based on casein, which in high concentrations, contributes to a delay in the process of sperm capacitation improving pregnancy rates.

Composition

Casein, sugars, amino acids, antioxidants, cholesterol, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

It is important to respect the dilutions from the table below. After dilution wait 15 minutes before use. In cases that centrifugation before cooling is necessary, resuspend with at least 100x10E6 sptz/mL.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

For better results in freezing semen, use BotuSemen Gold during centrifugation.

SPERMATIC CONCENTRATION
DILUTION (V:V)
SPERMATIC CONCENTRATION
DILUTION (V:V)
90x10E6/mL
1:1 Centrifuged Resuspended 100x10E6
170 to 210x10E6/mL
4:1
90 to 120x10E6/mL
2
He
Helium
130 to 160x10E6/mL
3
Li
Lithium

*Consult the Botupharma APP to do the dilution calculations.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuBOV

BotuBOV

Freezing extender for bulls and small ruminants

Designed to protect and preserve the sperm integrity during freezing process

Bovine Reproduction

Net contents:
100 mL

Description

Freezing extender for bulls and small ruminants.

More information

BotuBOV advanced formulation was developed to protect and preserve
the sperm integrity during the freezing process.


In recent studies, consistent data shows that BotuBOV out performs
other available extenders on the market and presents superior Fixed AI results.

Available

Net contents 100 mL

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

Red Cushion

RedCushion

Protects Sperm Cells During Centrifugation

Cushion fluid of high density

Equine Reproduction

Net contents:
20 mL and 100 mL

Red Cushion is a cushion fluid of high density used for semen centrifugation. It reduces damage from centrifugation through sperm damping over the Red Cushion on the bottom of the tube. It produces less compacted pellets with high sperm recovery rate due to the possibility of working with intensity and time of centrifugation above those used in conventional protocols. Because it has a reddish coloration, it allows easy visualization of the pellet and its removal.

Directions for usage (1):

  1. 1 – Aspire 3 mL of RedCushion with new syringe and needles, and deposit it at the bottom of the centrifugation tube;

    2 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v;

    3 – Incline the tube to 45º and slowly deposit the diluted semen on the wall with plastic Pasteur pipettes so that no mixing occurs between the semen and the RedCushion;

    4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm);

    5 – With help of a pipette remove the sperm “ring” deposited on top of RedCushion;

    6 – Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

Directions for usage (2):

  1. 1 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v;

    2 – Insert the diluted semen into a centrifuge tube;

    3 – With help of new syringe and needles deposit 3 mL of RedCushion under the diluted semen;

    4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm);

    5 – Remove the supernatant;

    6 – Aspire the formed pellet over the RedCushion;

    7 – Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

Storage and stability

Store it at 2-8ºC.

Available

Net contents 20 mL

Net contents 100 mL

Video how to use

Reproduzir vídeo

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

Equine Artificial Vagina

Equine Artificial Vagina

17” artificial vagina kit

Equine Reproduction

The Equine Artificial Vagina Kit consists of:

– High resistance rigid tube with polyurethane paint, with water inlet cock that also allows the air to be inflated, reducing the final weight of the vagina. The rigid tube has an anatomical design for better stallion comfort during semen collection.

 

– Latex Mucosa with 1.2 mm thickness and high durability.

 

– Disposable plastic mucosa for better hygiene during semen collection.

 

– Collecting cup coated with polyurethane, isothermal and protected from sunlight, providing ideal protection to the spermatozoa.

 

– Reusable bags that allow optimizing the use of the collection cup, maintaining the hygiene of the cup during the harvesting procedure.

Video how to set

Reproduzir vídeo

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm