EquiPure

equipure

Density Gradient product designed to increase the quality and viability of equine sperm

Equine Reproduction

Net contents:
100 mL

This is achieved by separating the sperm by density centrifugation prior to insemination, freezing, IVF or semen sexing. EquiPure not only eliminates a high proportion of the abnormal spermatozoa but also removes the source of reactive oxygen species, most bacteria and many viruses (EAV). This significantly increases the sperm survival and their fertilizing potential.

Features

– Improves fertility rates, especially in sub-fertile stallions

 

– Improves freezeability of equine sperm

 

– Removes bacteria and other unwanted components from raw ejaculates or inseminating doses.

 

-Removes Equine Arteritis Virus (EAV) particles

 

-Studies performed on the hygienic aspects of semen storage for AI demonstrate that the addition of antibiotics to extenders is often not enough to eliminate the detrimental influence of bacteria on sperm motility, viability and fertility. EquiPure centrifugation prior to freezing removes bacteria and many viruses (EAV).

 

-Poor quality frozen semen: If the sperm to be treated are already frozen, thaw at 37ºC the straws, and wait for approximately 10 minutes prior centrifugation. Then, put the same volume of EquiPure (proportion 1:1). Example: In a tube of 15 mL, first add 2 mL of EquiPure (Bottom) 4 straws (2 mL) of semen (TOP) centrifuge 400 xg 20 minutes. Finally, resuspend the pellet with 1 to 2 mL of BotuCRIO.

Available

Net contents 100 mL

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bOTUcrio

Integrity protection of sperm

BotuTURBO

BotuTurbo

Increase sperm motility extender

Developed to improve vigor of slow moving sperm

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuTURBO is an extender developed to improve vigor of slow moving sperm. It is highly recommended for fresh insemination and semen transport from stallions that need increased movement.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, pentoxifylline, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

For transport of cooled semen dilute to concetration of 30 to 50 million sperm per mL. Use 5ºC for semen transport. Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen. High recommended for reinforcement breeding (Thoroughbred).

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

*Consult the Botupharma APP to do the dilution calculations.

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bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuSEMEN GOLD

BotuSemen gold

High Fertility Semen Extender

casein + cholesterol

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSEMEN GOLD has been developed based on casein, which in high concentrations, contributes to a delay in the process of sperm capacitation improving pregnancy rates.

Composition

Casein, sugars, amino acids, antioxidants, cholesterol, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

It is important to respect the dilutions from the table below. After dilution wait 15 minutes before use. In cases that centrifugation before cooling is necessary, resuspend with at least 100x10E6 sptz/mL.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

For better results in freezing semen, use BotuSemen Gold during centrifugation.

SPERMATIC CONCENTRATION
DILUTION (V:V)
SPERMATIC CONCENTRATION
DILUTION (V:V)
90x10E6/mL
1:1 Centrifuged Resuspended 100x10E6
170 to 210x10E6/mL
4:1
90 to 120x10E6/mL
2
He
Helium
130 to 160x10E6/mL
3
Li
Lithium

*Consult the Botupharma APP to do the dilution calculations.

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bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

Red Cushion

RedCushion

Protects Sperm Cells During Centrifugation

Cushion fluid of high density

Equine Reproduction

Net contents:
20 mL and 100 mL

Red Cushion is a cushion fluid of high density used for semen centrifugation. It reduces damage from centrifugation through sperm damping over the Red Cushion on the bottom of the tube. It produces less compacted pellets with high sperm recovery rate due to the possibility of working with intensity and time of centrifugation above those used in conventional protocols. Because it has a reddish coloration, it allows easy visualization of the pellet and its removal.

Directions for usage (1):

  1. 1 – Aspire 3 mL of RedCushion with new syringe and needles, and deposit it at the bottom of the centrifugation tube;

    2 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v;

    3 – Incline the tube to 45º and slowly deposit the diluted semen on the wall with plastic Pasteur pipettes so that no mixing occurs between the semen and the RedCushion;

    4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm);

    5 – With help of a pipette remove the sperm “ring” deposited on top of RedCushion;

    6 – Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

Directions for usage (2):

  1. 1 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v;

    2 – Insert the diluted semen into a centrifuge tube;

    3 – With help of new syringe and needles deposit 3 mL of RedCushion under the diluted semen;

    4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm);

    5 – Remove the supernatant;

    6 – Aspire the formed pellet over the RedCushion;

    7 – Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

Storage and stability

Store it at 2-8ºC.

Available

Net contents 20 mL

Net contents 100 mL

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Integrity protection of sperm

BotuSEMEN

BotuSemen

Equine semen extender

Safe sperm transport

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSemen is an extender used to dilute the ejaculate during the centrifugation process for freezing. It has been developed after years of research. Its current formulation enables the sperm safe transportation until the moment of insemination.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

For transport of cooled semen dilute to concentration of 30 to 50 million sperm per mL. Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

*Consult the Botupharma APP to do the dilution calculations.

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bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuEMBRYO

BotuEmbryo

Maintenance and transport of embryos

Highly stable

Equine Reproduction

Net contents:
8 mL

BotuEmbryo is a media for maintenance and transport of embryos in field conditions. Its packing does not require the use of syringes and needles. It has an innovative formulation based on scientific studies carried out by national and international researchers.

 

BotuEmbryo is highly stable, allowing the embryo transport at environmental or refrigerated temperature in appropriate containers such as BotuFlex for up to 24 hours.

Storage and stability

Transport Temperature: Environment for up to 3 days.
Storage Temperature: Refrigerated at 2-8ºC.

Available

Net contents 8 mL

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bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation

BotuVITAL

Botuvital

Diluent for sperm evaluation

Eosin and Nigrosin based

Equine Reproduction

Net contents:
10 mL

BotuVital is a diluent for sperm evaluation based on Eosin and Nigrosin, which allows the analysis of the sperm pathologies as well as the visualization of plasma membrane integrity in light microscopy at 100x immersion objective.

How to use

  1. Over one of the slide ends, deposit 1 drop of fresh semen (free of diluent) and 1 drop of BotuVital;
  2. Homogenize the drops until getting a mixture of uniform coloring;
  3. Smear with help of another slide, slightly loosening the uniform mixture in order to obtain a very thick and dark smear and do not cause mechanical injuries to the sperm, according to the scheme below:
  1. Wait 5 minutes for the mixture to dry;
  2. Read in light microscopy at 100x magnification (immersion);
  3. Count 200 sperm to measure the spermatic pathologies together with stained and unstained ones:

– Sperms stained in pink show damaged plasma membrane.
– Sperms with absence of staining show full plasma membrane.

Storage and stability

Store it in a dry or cool environment.

Available

Net contents 10 mL

Video how to use

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Integrity protection of sperm

BotuCRIO

Botucrio

Integrity protection of sperm

FREEZING OF EQUINE SEMEN

Equine Reproduction

Net contents:
25 mL

Composition

Sugars, antioxidants, amino acids, egg yolk, cryoprotectant, and vehicle.

Storage and stability

Store it in a freezer at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days. Add the contents of the bottle directly into the centrifuged semen.

Available

Net contents 25 mL

BotuCRIO has in its formulation innovative substances to protect the sperm integrity. BotuCRIO combines the best components to protect cells from damage caused by freezing and to protect their fertility after thawing.

 

BotuCRIO offers the best motility parameters. The formulation of BotuCRIO results in best integrity, speed and viability of the sperm. In addition, it provides better motility after total and progressive thawing compared to other commercially available freezing extenders.

 

BotuCRIO shows the best fertility rates. Studies indicate that frozen stallion sperm with BotuCRIO have better fertility compared to other extenders. These in vitro results were confirmed during field experiments by providing higher pregnancy rates per cycle after insemination.

 

In international studies, BotuCRIO when compared to other freezing extenders always presented the best results of freezing and fertility. BotuCRIO is the most used semen extender in the world!

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Broodmare PLUS

For the improvement of oocyte metabolic

function and embryonic development

STALLION PLUS

Liquid vitamin,  mineral, amino acid,  and

fatty acid supplement for horses.