EquiPure

equipure

Density Gradient product designed to increase the quality and viability of equine sperm

Equine Reproduction

Net contents:
100 mL

This is achieved by separating the sperm by density centrifugation prior to insemination, freezing, IVF or semen sexing. EquiPure not only eliminates a high proportion of the abnormal spermatozoa but also removes the source of reactive oxygen species, most bacteria and many viruses (EAV). This significantly increases the sperm survival and their fertilizing potential.

Features

– Improves fertility rates, especially in sub-fertile stallions

 

– Improves freezeability of equine sperm

 

– Removes bacteria and other unwanted components from raw ejaculates or inseminating doses.

 

-Removes Equine Arteritis Virus (EAV) particles

 

-Studies performed on the hygienic aspects of semen storage for AI demonstrate that the addition of antibiotics to extenders is often not enough to eliminate the detrimental influence of bacteria on sperm motility, viability and fertility. EquiPure centrifugation prior to freezing removes bacteria and many viruses (EAV).

 

-Poor quality frozen semen: If the sperm to be treated are already frozen, thaw at 37ºC the straws, and wait for approximately 10 minutes prior centrifugation. Then, put the same volume of EquiPure (proportion 1:1). Example: In a tube of 15 mL, first add 2 mL of EquiPure (Bottom) 4 straws (2 mL) of semen (TOP) centrifuge 400 xg 20 minutes. Finally, resuspend the pellet with 1 to 2 mL of BotuCRIO.

Available

Net contents 100 mL

Video how to use

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BotuTURBO

BotuTurbo

Increase sperm motility extender

Developed to improve vigor of slow moving sperm

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuTURBO is an extender developed to improve vigor of slow moving sperm. It is highly recommended for fresh insemination and semen transport from stallions that need increased movement.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, pentoxifylline, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

For transport of cooled semen dilute to concetration of 30 to 50 million sperm per mL. Use 5ºC for semen transport. Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen. High recommended for reinforcement breeding (Thoroughbred).

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

*Consult the Botupharma APP to do the dilution calculations.

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BotuSEMEN SPECIAL

BotuSemen special

Maximum Protection for Sperm

with cholesterol

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSEMEN SPECIAL an extender especially developed for stallions with semen sensitive to cooling process. Its composition is based on the addition of essential lipids to maintain the plasma membrane stability of the sperm during the cooling process.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, cholesterol and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

It is important to respect the dilutions from the table below. After dilution wait 15 minutes before use. In cases that centrifugation before cooling is necessary, resuspend with at least 100x10E6 sptz/mL.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

For better results in freezing semen, use BotuSemen Gold during centrifugation.

SPERMATIC CONCENTRATION
DILUTION (V:V)
SPERMATIC CONCENTRATION
DILUTION (V:V)
90x10E6/mL
1:1 Centrifuged Resuspended 100x10E6
170 to 210x10E6/mL
4:1
90 to 120x10E6/mL
2
He
Helium
130 to 160x10E6/mL
3
Li
Lithium

*Consult the Botupharma APP to do the dilution calculations.

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Integrity protection of sperm

bOTUvital

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BotuSEMEN GOLD

BotuSemen gold

High Fertility Semen Extender

casein + cholesterol

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSEMEN GOLD has been developed based on casein, which in high concentrations, contributes to a delay in the process of sperm capacitation improving pregnancy rates.

Composition

Casein, sugars, amino acids, antioxidants, cholesterol, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

It is important to respect the dilutions from the table below. After dilution wait 15 minutes before use. In cases that centrifugation before cooling is necessary, resuspend with at least 100x10E6 sptz/mL.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

For better results in freezing semen, use BotuSemen Gold during centrifugation.

SPERMATIC CONCENTRATION
DILUTION (V:V)
SPERMATIC CONCENTRATION
DILUTION (V:V)
90x10E6/mL
1:1 Centrifuged Resuspended 100x10E6
170 to 210x10E6/mL
4:1
90 to 120x10E6/mL
2
He
Helium
130 to 160x10E6/mL
3
Li
Lithium

*Consult the Botupharma APP to do the dilution calculations.

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Red Cushion

RedCushion

Protects Sperm Cells During Centrifugation

Cushion fluid of high density

Equine Reproduction

Net contents:
20 mL and 100 mL

Red Cushion is a cushion fluid of high density used for semen centrifugation. It reduces damage from centrifugation through sperm damping over the Red Cushion on the bottom of the tube. It produces less compacted pellets with high sperm recovery rate due to the possibility of working with intensity and time of centrifugation above those used in conventional protocols. Because it has a reddish coloration, it allows easy visualization of the pellet and its removal.

Directions for usage (1):

  1. 1 – Aspire 3 mL of RedCushion with new syringe and needles, and deposit it at the bottom of the centrifugation tube;

    2 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v;

    3 – Incline the tube to 45º and slowly deposit the diluted semen on the wall with plastic Pasteur pipettes so that no mixing occurs between the semen and the RedCushion;

    4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm);

    5 – With help of a pipette remove the sperm “ring” deposited on top of RedCushion;

    6 – Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

Directions for usage (2):

  1. 1 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v;

    2 – Insert the diluted semen into a centrifuge tube;

    3 – With help of new syringe and needles deposit 3 mL of RedCushion under the diluted semen;

    4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm);

    5 – Remove the supernatant;

    6 – Aspire the formed pellet over the RedCushion;

    7 – Resuspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.

Storage and stability

Store it at 2-8ºC.

Available

Net contents 20 mL

Net contents 100 mL

Video how to use

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Equine Artificial Vagina

Equine Artificial Vagina

17” artificial vagina kit

Equine Reproduction

The Equine Artificial Vagina Kit consists of:

– High resistance rigid tube with polyurethane paint, with water inlet cock that also allows the air to be inflated, reducing the final weight of the vagina. The rigid tube has an anatomical design for better stallion comfort during semen collection.

 

– Latex Mucosa with 1.2 mm thickness and high durability.

 

– Disposable plastic mucosa for better hygiene during semen collection.

 

– Collecting cup coated with polyurethane, isothermal and protected from sunlight, providing ideal protection to the spermatozoa.

 

– Reusable bags that allow optimizing the use of the collection cup, maintaining the hygiene of the cup during the harvesting procedure.

Video how to set

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SpermFILTER

BEFORE
AFTER

spermfilter

Sperm concentrator filter

Synthetic membrane filter

Equine Reproduction

SpermFilter is a synthetic membrane filter with porosity that allows the passage of seminal plasma and bacteria, retaining only sperms. The filter eliminates the need of a centrifuge, being less harmful to sperm when compared to the centrifugation usually used to concentrate sperm.

 

Carefully remove the filter from the package. Place the Petri dish on a surface heated to a temperature of 30-35ºC. Dilute the semen with BotuSEMEN and pour it into the SpermFILTER. To concentrate the sperm, gently swirl the diluted semen around the filter and repeatedly touch the bottom of filter on the bottom of Petri dish until only a slight amount of liquid is evident on the filter membrane.

 

Wash the filter with appropriate volume of either BotuSEMEN or BotuCRIO extender. Repeat swirling motions to resuspend the sperm and pour it into a beaker.

If sperm recovery is low due to smaller size of the sperm, it may be necessary to refilter the ejaculate. The SpermFILTER can be reused for the same stallion up to 10 times. Wash it with sterile distilled water and dry at 37ºC.

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BotuFLEX

Botuflex

Safe transport of semen and embryo

The best system in the market designed for embryo transport

Equine Reproduction

AVAILABLE: 
Styrofoam box containing three units of BotuIA, and two ice pack.
Optional: Adapter to 50 mL Falcon Tube

BotuFLEX is a low cost system developed to achieved adequate cooling curves both at 15ºC and 5ºC. Its sealing and density allow the shipped samples to reach the final destination at the appropriate temperature, even in adverse conditions of external temperatures.

 

Proven results  an high pregnancy rates.
 
Semen transport (2 ice pack)
Embryo transport (1 ice pack)

How to use

– BotuFlex should be set up on the time of use;

– Do not introduce new samples while the BotuFlex is in use;

– The cold sources (ices) should be frozen at -20ºC for at least 24 hours before each use;

– ALWAYS respect the quantity of 150 to 200 mL of volume per shipping, if necessary use a bottle of water with the missing volume to complete the 150 to 200 mL;

– The BotuFlex can work at 15ºC (using 1 ice) or 5ºC (using 2 ice);

– For transports until 12 hours use 1 or 2 ice, above 12 hours always use 2 ice;

– For best results in refrigerated transport, use BotuSemen extender;

– For stallions with low motility, try BotuTurbo extender;

– With BotuTurbo extender, always use the 5ºC curve (using 2 ice);

– Do not open the BotuFlex before final destination;

– The refrigeration curves with 1 or 2 ice are represented at the graphs below:

Available

Styrofoam box containing three units of BotuIA, and two ice pack.
Optional: Adapter to 50 mL Falcon Tube

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bOTUai

For artificial insemination

BotuAI

Botuai

For artificial insemination

Transport of cooled semen

Equine Reproduction

BotuAI is a bottle for transport of cooled semen, which provides greater safety, practicality, and facility to identification of the shipped samples.

 

It avoids the use of plastic bags that in the most time can romper during transport causing loss of the transported sample.

 

It has a cover with design that allows to directly attaching the bottle to the pipette, dispensing the use of syringes for Artificial Insemination (AI).

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Botufelx

Safe transport of semen and embryo

BotuSEMEN

BotuSemen

Equine semen extender

Safe sperm transport

Equine Reproduction

AVAILABLE:
Pack of 10 diluent bottles and 10 sachets with 8.5 g each our

1 diluent bottle and 1 sachet with 17 g.

BotuSemen is an extender used to dilute the ejaculate during the centrifugation process for freezing. It has been developed after years of research. Its current formulation enables the sperm safe transportation until the moment of insemination.

Composition

Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, and excipients.

How to prepare

Add all the content of this packet in sterile water and shake very well.

Available

Pack of 10 diluent bottles and

10 sachets with 8.5 g each

1 diluent bottle 200 mL

and 1 sachet with 17 g.

How to use

For transport of cooled semen dilute to concentration of 30 to 50 million sperm per mL. Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen.

Storage and stability

After mixing, store it at 2-8ºC for up to 3 days.

*Consult the Botupharma APP to do the dilution calculations.

More product information
and where to find it

Discover more Botupharma USA products

Discover more

Botupharma USA products

bOTUcrio

Integrity protection of sperm

bOTUvital

Diluent for sperm evaluation