Description
EQUINE SEMEN EXTENDER FOR SAFE SPERM TRANSPORT
Botusemen is an extender developed after years of research, and its current formulation enables the safe transport of spermatozoa until the time of insemination or during the centrifugation process for freezing protocols. Suitable for most stallions.
How to Use
How to prepare
Add the sachet’s content to the sterile water and shake well.
How to use
For cooled transport, semen should be refrigerated at 5 °C (41 °F).
The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL, as shown in the table below:
SPERM CONCENTRATION |
DILUTION (V:V) |
< 60 million sperm/ mL |
Centrifuge or filter with Sperm Filter |
70 to 90 million sperm/ mL
|
|
100 to 200 million sperm/ mL
|
|
210 to 250 million sperm/ mL
|
|
260 to 300 million sperm/ mL
|
|
For artificial insemination with fresh semen or centrifugation for freezing protocols,
dilute 1 part extender to 1 part semen (1:1; V:V). After semen dilution, wait 15 minutes to proceed with artificial insemination.