BotuSemen Special is an extender especially developed for stallions with semen sensitive to cooling process. Its composition is based on the addition of essential lipids to maintain the plasma membrane stability of the sperm during the cooling process.
Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, cholesterol and excipients.
HOW TO PREPARE:
Add all the content of this packet in 100 mL of sterile water and shake very well.
HOW TO USE:
It is important to respect the dilutions from the table below. After dilution wait 15 minutes before use. In cases that centrifugation before cooling is necessary, resuspend with at least 100x10E6 sptz/mL.
STORAGE AND STABILITY:
After mixing, store it at 2-8ºC for up to 3 days.
|Spermatic Concentration||Dilution (v:v)||Spermatic Concentration||Dilution (v:v)|
|< 90x10E6/mL||1:1 Centrifuged Resuspended 100x10E6||170 to 210x10E6/mL||4:1|
|90 to 120x10E6/mL||2:1||220 to 250x10E6/mL||5:1|
|130 to 160x10E6/mL||3:1||260 to 300x10E6/mL||6:1|
For better results in freezing semen, use BotuSemen Special during centrifugation. *Consult the Botupharma APP to do the dilution calculations.