BotuCRIO

Equine Frozen Semen Extender Designed to Protect Sperm
Integrity in the Freezing Process
 
Net Contents: 25 mL

Description

BotuCRIO has in its formulation innovative substances to protect the sperm integrity. BotuCRIO combines the best components to protect cells from damage caused by freezing and to protect their fertility after thawing.

BotuCRIO offers the best motility parameters. The formulation of BotuCRIO results in best integrity, speed and viability of the sperm. In addition, it provides better motility after total and progressive thawing compared to other commercially available freezing extenders.

BotuCRIO shows the best fertility rates. Studies indicate that frozen stallion sperm with BotuCRIO have better fertility compared to other extenders. These in vitro results were confirmed during field experiments by providing higher pregnancy rates per cycle after insemination.

In international studies, BotuCRIO when compared to other freezing extenders always presented the best results of freezing and fertility. BotuCRIO is the most used semen extender in the world!

STORAGE AND STABILITY:
Store it in a freezer at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days. Add the contents of the bottle directly into the centrifuged semen.

 

Additional information

Weight 0.038 kg
Dimensions 1.57 × 1.57 × 2.36 cm

How to Use

AFTER SEMEN COLLECTION:
– Determine sperm parameters: volume, motility and concentration.
– Dilute the ejaculate with BotuSemen at 1:1 proportion (V:v).
– Centrifuge at regular 600x G for 10 minutes or with Red Cushion 1000x G for 20 minutes.
– Remove supernatant.
– Resuspend pellet with BotuCrio to 200×10 sperm/ml or to 100×10 sperm/straw.
– Pack and seal straws.
– Leave straws into the refrigerator at 5°C (Celsius) for 20 to 30 minutes.
– Add 2,5 inches (6 cm) of nitrogen into Styrofoam box.
– Put straws 1.2 inches (3 cm) to 2.4 inches (6 cm) above the liquid nitrogen level for 20 minutes.
– Plunge the straws directly into the nitrogen liquid.
– Thaw straws in warming water bath at 37°C for 30 seconds or at 46°C for 20 seconds.

FROZEN MACHINE:
– Set machine to 5°C (Celsius)
– Add straws to machine and cool at 5°C for 20 to 30 minutes.
– Start the negative rates around -60°C to -30°C per minute until at least-120°C.
– Plunge the straws directly into the nitrogen liquid.
– Thaw straws in warming water bath at 37°C for 30 seconds or vat 46°C for 20 seconds.

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BotuCRIO

Equine Frozen Semen Extender Designed to Protect Sperm
Integrity in the Freezing Process
 
Net Contents: 25 mL

Description

BotuCRIO has in its formulation innovative substances to protect the sperm integrity. BotuCRIO combines the best components to protect cells from damage caused by freezing and to protect their fertility after thawing.

BotuCRIO offers the best motility parameters. The formulation of BotuCRIO results in best integrity, speed and viability of the sperm. In addition, it provides better motility after total and progressive thawing compared to other commercially available freezing extenders.

BotuCRIO shows the best fertility rates. Studies indicate that frozen stallion sperm with BotuCRIO have better fertility compared to other extenders. These in vitro results were confirmed during field experiments by providing higher pregnancy rates per cycle after insemination.

In international studies, BotuCRIO when compared to other freezing extenders always presented the best results of freezing and fertility. BotuCRIO is the most used semen extender in the world!

STORAGE AND STABILITY:
Store it in a freezer at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days. Add the contents of the bottle directly into the centrifuged semen.

 

Additional information

Weight 0.038 kg
Dimensions 1.57 × 1.57 × 2.36 cm

How to Use

AFTER SEMEN COLLECTION:
– Determine sperm parameters: volume, motility and concentration.
– Dilute the ejaculate with BotuSemen at 1:1 proportion (V:v).
– Centrifuge at regular 600x G for 10 minutes or with Red Cushion 1000x G for 20 minutes.
– Remove supernatant.
– Resuspend pellet with BotuCrio to 200×10 sperm/ml or to 100×10 sperm/straw.
– Pack and seal straws.
– Leave straws into the refrigerator at 5°C (Celsius) for 20 to 30 minutes.
– Add 2,5 inches (6 cm) of nitrogen into Styrofoam box.
– Put straws 1.2 inches (3 cm) to 2.4 inches (6 cm) above the liquid nitrogen level for 20 minutes.
– Plunge the straws directly into the nitrogen liquid.
– Thaw straws in warming water bath at 37°C for 30 seconds or at 46°C for 20 seconds.

FROZEN MACHINE:
– Set machine to 5°C (Celsius)
– Add straws to machine and cool at 5°C for 20 to 30 minutes.
– Start the negative rates around -60°C to -30°C per minute until at least-120°C.
– Plunge the straws directly into the nitrogen liquid.
– Thaw straws in warming water bath at 37°C for 30 seconds or vat 46°C for 20 seconds.

Reviews

There are no reviews yet.

Be the first to review “BotuCRIO”

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